Analyze, graph and present your scientific work.
Prism è
un ottimo software per la biostatistica di base e la grafica
scientifica.
Grazie alla semplicità di approccio, alla potenza di
analisi e di grafica negli esperimenti ripetitivi, all'applicazione
del test statistico più appropriato, alla capacità
di interpretazione dei risultati, Prism è il software
ideale per l'applicazione nelle scienze della vita.
Quello
che rende Prism unico non è tanto quello che fa, ma
come lo fa.
Creato per i ricercatori di laboratorio e clinici, Prism guida
l'utilizzatore privo di conoscenze statistiche attraverso
il processo di analisi, supportandolo con tutto l'aiuto
che necessita; è possibile così concentrarsi
sui dati e non sul funzionamento del software. 

Why Choose Prism?
Save Time Performing Statistical Analyses
A versatile statistics
tool purposebuilt for scientists—not statisticians. Get a head start by
entering data into tables that are structured for scientific research
and guide you to statistical analyses that streamline your research
workflow. No coding required.
Learn more 

Make More Accurate, More Informed Analysis Choices
Gain insights and guidance
at every step so you make the right analysis choices, understand the
underlying assumptions, and accurately interpret your data along the
way.
Learn more 

Tell a Story With Your Data
Prism makes it easy to
collaborate with colleagues, receive feedback from peers, and share your
research with the world. Go from data to elegant, publicationquality
graphs—with ease. Prism offers countless ways to customize your graphs,
from color schemes to how you organize data. Export into almost any
format, send to PowerPoint, or email directly from the application.
Learn more 

Introducing Prism 8!
The most intuitive and sophisticated version of Prism yet.
Learn More Resources
Master the key principles of statistics with GraphPad's hugely popular educational guides and resources.



Statistics
help you understand patterns in the world. But analyzing data
incorrectly can result in misleading or false conclusions when
interpreting those patterns. This guide will help you avoid common
mistakes that many textbooks barely mention. 
Many
scientists fit curves more often than they use any other statistical
technique, yet many don’t really understand the principles. This guide
provides a concise introduction to fitting curves, especially nonlinear
regression. 
Join the thousands of scientists that use one of our 25+ online calculators to run quick statical tests every day. 
The preferred analysis and graphing solution purposebuilt for
scientific research. Join the world's leading scientists and discover
how you can use Prism to save time,
make more appropriate analysis choices, and elegantly graph and
present your scientific research.
Comprehensive Analysis and Powerful Statistics, Simplified
Organize Your Data Effectively
Unlike spreadsheets or other scientific graphing programs, Prism
has eight different types of data tables specifically formatted for the
analyses you want to run. This
makes it easier to enter data correctly, choose suitable
analyses, and create stunning graphs. 

Perform The Right Analysis
Avoid statistical jargon. In clear language, Prism presents an
extensive library of analyses from common to highly specific—nonlinear
regression, t tests, nonparametric
comparisons, one, two and threeway ANOVA, analysis of
contingency tables, survival analysis, and much more. Each analysis has a
checklist to help you understand the
required statistical assumptions and confirm you have selected
an appropriate test. 

Get Actionable Help As You Go
Reduce the complexity of statistics. Prism’s online help goes
beyond your expectations. At almost every step, access thousands of
pages from the online Prism Guides.
Browse the Graph Portfolio and learn how to make a wide range of
graph types. Tutorial data sets also help you understand why you should
perform certain analyses and
how to interpret your results. 

Work Smarter, Not Harder
OneClick Regression Analysis
No other program simplifies curve fitting like Prism. Select an
equation and Prism does the rest—fits the curve, displays a table of
results and function parameters,
draws the curve on the graph, and interpolates unknown values. 

Focus on Your Research, Not Your Software
No coding required. Graphs and results are automatically updated
in real time. Any changes to the data and analyses—adding missed data,
omitting erroneous data,
correcting typos, or changing analysis choices—are reflected in
results, graphs, and layouts instantaneously. 

Automate Your Work Without Programming
Reduce tedious steps to analyze and graph a set of experiments.
It is easy to replicate your work by creating a template, duplicating a
family, or cloning a
graph—saving you hours of set up time. Apply a consistent look
to a set of graphs with one click using Prism Magic. 

The Fastest Way to Elegantly Graph and Share Your Work
Countless Ways to Customize Your Graphs
Focus on the story in your data, not manipulating your software.
Prism makes it easy to create the graphs you want. Choose the type of
graph, and customize any parthow
the data is arranged, the style of your data points, labels,
fonts, colors, and much more. The customization options are endless.
Export PublicationQuality Graphs With One Click
Reduce time to publish. Prism allows you to customize your
exports (file type, resolution, transparency, dimensions, color space
RGB/CMYK) to meet the requirements of
journals. Set your defaults to save time. 

Enhance Collaboration
Share more than your graphs. Prism’s comprehensive record of
your data enables effective collaboration with other scientists. All
parts of your Prism project (raw data,
analyses, results, graphs, and layouts) are contained in a
single file that you can share with one click. Now others can easily
follow your work at every step,
enhancing the clarity of your findings and streamlining your
collaborative efforts.


Prism 8.3.0 Release Notes
Introducing Logistic Regression
You've been asking for it, and we've delivered: Simple Logistic
Regression and Multiple Logistic Regression are now available in Prism
8.3.0!
A quick overview
One of the most toprequested features has continuously been the
ability to perform logistic regression with Prism. In many experiments,
your measurement of interest can only take on one of two possible
outcomes: yes or no, 1 or 0, "event occurred" or "event didn't occur".
In these situations, the right analysis for the job is often logistic
regression, and with the release of Prism 8.3.0, we're very excited
to announce that this analysis is now available to you. In fact, Prism
8.3.0 actually introduces two separate  but very similar  analyses:
Simple Logistic Regression
Multiple Logistic Regression
Stay connected
Your feedback and suggestions made logistic regression in Prism a
reality. Do you have other ideas for new features? Suggestions for
improvements to existing features? Email us at suggestions@graphpad.com to let us know!
Complete Release Notes for Prism 8.3.0
New Features
 Implemented Simple logistic regression from XY data tables
 Implemented Multiple logistic regression from Multiple variables data tables
Feature Improvements
 Added an alert regarding data mismatch for 'Oneway ANOVA (and
nonparametric or mixed)' analysis if data sets were excluded resulting
in fewer than three data sets in the analysis
 Updated the name of the 'Linear regression' analysis to 'Simple linear regression'
 Added analytical representations of polynomial functions (5th, 6th, and 10th order)
 Improved autorecovery of Prism files under specific situations
Performance (Speed) Improvements
 Improved performance of nonlinear regression that uses GaddumSchild equations
 (Windows) Improved performance of copying and pasting data sets with a huge number of rows
 (Windows) Improved performance of importing TXT data
 (Windows) Improved performance of selecting thumbnails in the combined gallery view of Data+results sheets
 (Mac) Improved performance when first switching to 'Open a File' tab on the Welcome dialog
 (Mac) Improved Navigator performance for large projects
Analysis Bug Fixes
 Fixed the issue in which the onesample ttest produced empty
results for replicated data if each subcolumn was analyzed separately
 Fixed the issue in which LogEC50 and HillSlope results were
replaced with "???" and the results for EC50 were empty when using the
"Identify "unstable" parameters" option
 Fixed the issue in which LOWESS/Smoothing splines would generate incorrect residuals when input XY table contained replicates
 Fixed the Y title for the residual plot generated from Fit spline/LOWESS analysis
 Improved the accuracy of interpolation on the bounds of the data range using Akima splines
 Fixed the issue in which Prism would crash when performing
"Transform" analysis with Rank Y transform and interchange XY, while one
column contained a title but no data
 Fixed the issue in which Normalize each sub column separately could
not be selected in the Normalize dialog if the first data set was
deleted from the source data table
 Added a link to the Curve Fitting Guide from the descriptions of
analyses of "Doseresponse  Special, X is concentration" family
 (Windows) Fixed the issue in which the "Subcolumns" section of the
"Parameters: Normalize" dialog was unexpectedly greyed out if the first
data set of the source data table was empty
 (Windows) Fixed issue in which Prism would crash when performing
'Multiple linear regression' after adding more than 256 components to
the model
 (Windows) Fixed the issue in which "β0" was reported in the "Hook
Constant" dialog if "Multiple Linear Regression" analysis was performed
without an intercept
 (Windows, 32bit only) Fixed the issue in which Prism would crash
while changing parameters for Twoway ANOVA when the result sheet was
orphaned
 (Mac) Fixed the issue in which the 'Geo. mean of ratios CI plot'
results sheet of a t test appeared as "Mean, Upper Limit/Lower Limit"
instead of "Geometric Mean/Upper Limit/Lower Limit"
 (Mac) Fixed the issue in which 'narrative results' appeared
corrupted after all source data sets except the first were deleted,
followed by using the "Undo" operation
 (Mac) Fixed the issue in which the "Number of segments" value was
unexpectedly reset from a hooked value to the default in the "Fit
Spline/LOWESS" dialog
 (Mac) Updated links for the "Help" button in the "Parameters: Fit
Spline/LOWESS" dialog and corresponding "Interpret" toolbar button
 (Mac) Fixed the issue in which the "MannWhitney test" and
"KolmogorovSmirnov test" radio buttons were incorrectly displayed after
toggling between options in the t test dialog
 (Mac) Renamed the tab in the "Parameters: Simulate XY data" dialog
to "Parameter values & column titles" instead of "Parameter values"
Graphing Bug Fixes
 Fixed the issue in which incorrect labels were displayed on the X
axis of a Nested graph if data had been excluded or deleted from the
data table
 Fixed the issue in which long legends on a graph became disrupted in Mac Prism projects opened on Windows
 Fixed the issue in which graph axes and labels would appear shifted from their positions after reopening the project
 Fixed the issue in which graph axes and labels would shift from their positions when page orientation was changed
 (Windows) Fixed the issue in which error bars on a column bar graph
unexpectedly appeared thinner near the range limit of the axis
 (Windows) Removed an unexpected thin gap between data sets on a
Grouped graph when the settings for the "gap between adjacent data" was
set to 0%
 (Windows) Fixed the issue in which the left side of the graph
appeared cut off on a layout sheet after deleting the Y axis title and
changing the row label's font style
 (Windows) Fixed the issue in which axes grid lines partially
disappeared for one segment if the right Y axis and X axis were split
into three segments
 (Mac) Made it possible to change the color of spike bars using the Format Points contextual menu from a data table
 (Mac) Fixed the issue in which the tooltip for heat map legends displayed truncated values
 (Mac) Fixed the issue in which data point symbols on a graph
unexpectedly appeared in a color different from the curve after adding
transformed data to the graph and applying a color scheme
 (Mac) Fixed the issue in which custom symbols which were taken from
the "Character Palette" dialog lost their formatting after entering any
text in the row title of the source data table
 (Mac) Fixed the issue in which data sets that were added from a
different data table to "LowHigh", "Box & Whiskers", or "Violin"
graphs from the Nested family incorrectly appeared as bars
 (Mac) Fixed the issue in which the format of axes legends on a heat
map were not applied after changing it from the "Powers of ten" option
to "Decimal"
 (Mac) Fixed the issue in which the default format for the right Y
axis was incorrect if the "Ln (Natural Log)" scale was applied to the
left Y axis
 (Mac) Fixed the issue in which a heat map that was created from
Correlation Matrix analysis appeared almost entirely black when the
source data table contained a large number of data sets
 (Mac) Fixed the issue in which vertical bar graphs with a custom
baseline appeared distorted after changing the graph type to horizontal
 (Mac) Fixed the issue in which annotation labels on a graph were cut if there was a gap in the axis
 (Mac) Fixed the issue in which some error bars retained a gap  if
the graph they were plotted on previously used a segmented axis  after
the "Discontinuous axis" checkbox was unchecked in the Format Graph
dialog
Other Bug Fixes
 Fixed the issue in which Prism would crash when attempting to write over 257 symbols to Info Notes using a Prism script
 Removed "ExportPCX" script command from the "Script Syntax" section of "GraphPad Prism Script" dialog
 Fixed the link to the Curve Fitting Guide from the description of
"Absolute IC50, X is log..." in the "Parameters: Nonlinear regression"
dialog
 Fixed the URL to the beta test page in the alert about beta expiration
 Removed QQ plot as an option for Poisson regression in the "Parameters: Multiple Linear Regression" dialog
 Improved floating note text: "This analysis was performed by a
newer version of Prism, so it cannot be recalculated and it will not
update when linked data are changed. Saving this file will result in
parameters being lost if this file is reopened in its original (newer)
version."
 Fixed the issue in which silent activation was not performed when a nonexpired license existed
 (Windows) Fixed the issue in which a range of highlighted cells was not updated accordingly after inserting a row or column
 (Windows, 32bit only) Fixed the issue in which Prism would crash
upon opening very large projects, and added an alert about the inability
of the 32bit edition to open projects containing too many data points
 (Windows) Fixed the issue in which Prism would hang after trying to
check the "Threeway interactions" checkbox in the "Parameters:
Multiple Linear Regression" dialog if the source data contained a huge
amount of data
 (Windows) Fixed the issue in which Prism would hang when switching
to the "Compare" tab of the "Parameters: Multiple Linear Regression"
dialog if the source data contained a huge number of columns
 (Windows) Fixed the issue in which Prism would crash while
performing Multiple Linear Regression with more than 100 independent
variables
 (Windows) Fixed the issue in which Prism would crash after breaking
the link to an XLS file from the "Links" dialog if the data table
contained data linked to a results sheet
 (Windows) Fixed the issue in which Prism would crash after trying to delete all graphs, except for one from gallery mode
 (Windows) Fixed the issue in which values could not be entered into
the default cell of a new data table created from the "Data+Results"
folder in the Navigator
 (Windows) Fixed the issue in which thumbnails of results sheets
became duplicated after deleting the source data table linked to at
least three chained analyses in combined "Data+Results" view in the
Navigator
 (Windows) Fixed the issue in which Prism returned to gallery mode
instead of sheet view when you double click a nonselected sheet from
the gallery
 (Windows) Fixed the issue in which the "Disk or memory error
occurred" alert unexpectedly appeared in an attempt to paste transpose
the data into the column title cell
 (Windows) Fixed the issue in which the "Disk or memory error
occurred" alert unexpectedly appeared when doubleclicking in the
graphing area of a "Parts of Whole" graph after its data had been
deleted
 (Windows) Fixed the issue in which the name of a graph sheet was
unexpectedly changed after editing a linked column title from a data
table with brackets [ ] in its name
 (Windows) Fixed the issue in which cells copied from MS Excel and
pasted into Prism as column titles did not affect the linked graph sheet
name
 (Windows) Fixed the issue in which Prism's log file generated by executing Prism's script appeared empty
 (Windows) Fixed the issue in which Prism would crash while attempting to add a column title from the "Format Data Table" dialog
 (Windows) Fixed the issue in which Prism would crash after
switching to a Heat map which was created from the Correlation Matrix
analysis if the source data contained a large number of data sets
 (Windows) Fixed the issue in which Prism would crash when pressing the CTRL+L shortcut while no project was open
 (Windows) Fixed the issue in which the names of all results sheets
in the Data+results folder unexpectedly became bolded on reordering by
dragging them in the Navigator
 (Windows) Fixed the issue in which artifacts would appear on XY data tables with empty error cells during vertical scrolling
 (Windows) Fixed the issue in which special characters were lost
from linked info tables after performing the "Duplicate Family"
operation twice
 (Windows) Fixed the issue in which the "Run simulation again"
toolbar button was absent for the "Simulate contingency table" results
sheet
 (Windows) Fixed the issue in which a graph thumbnail was not
updated in the gallery after making any changes in the "Format Graph"
dialog invoked from the toolbar
 (Windows) Fixed the issue in which an info hint on the bottom
toolbar did not change position and was truncated while resizing the
Navigator
 (Windows) Fixed the issue in which a results sheet was duplicated
in "Go To Linked Sheet" contextual menu invoked from the Navigator
 (Windows) Fixed the issue in which characters were ignored on
pasting multiword titles to column or subcolumn titles in the "Format
Data Table" dialog
 (Windows) Fixed the issue in which it was impossible to paste a
single row of column or subcolumn titles in the Column (or Subcolumn)
Titles tab of the "Format Data Table" dialog
 (Windows) Fixed the "Format Graph" dialog look so that the edit
field of the Baseline in the dialog was not corrupted when hovering the
mouse over it
 (Windows) Fixed the issue in which the "Line" formatting commands
in the "Format points" and toolbar button dropdowns were mistakenly
enabled for the "Scatter plot with bar" graph type
 (Windows) Fixed the issue in which the graph preview was incorrect
for vertical bar graphs with a custom baseline that had their graph type
changed to horizontal
 (Windows) Fixed the issue in which selection checkboxes on
thumbnails in gallery mode were not scaled properly on high DPI displays
 (Windows) Fixed the issue in which the "Hook" icon woudl disappear in the "Hook Constant" dialog on high DPI displays
 (Windows) Fixed the issue in which elements of the Navigator would
become corrupted and would overlap in the combined "Data+Results" folder
view on high DPI displays
 (Windows) Fixed the alignment of radio buttons in the "Measurement units" section of the Preferences dialog
 (Windows) Fixed the issue in which unexpected symbols were shown in data set names in the "Select Data Sets" dialog
 (Windows) Disabled the "Equalize scaling factor" toolbar button and menu item when there was a single graph on a layout
 (Mac) Fixed the issue in which Prism would crash after switching to
the graph sheet if the source data table was previously deleted and the
undo operation was performed
 (Mac) Fixed the issue in which Prism would crash while applying
shortcuts for switching tabs (CMD+1/2/3...) in the results gallery after
switching from sheet view for analysis with tabs
 (Mac) Fixed the issue in which the wrong graph sheet is displayed
after clicking the "Go To" button in the "Place Graph on Layout" dialog
 (Mac) Fixed the issue in which an endless calculation process was
shown after opening a file saved on a graph due to Prism mistakenly
trying to show the "Change Graph Type" dialog while an analysis plotted
on the graph was being recalculated
 (Mac) Fixed the issue in which Prism unexpectedly displayed a different sheet after changing the sheet order in the Navigator
 (Mac) Fixed the issue in which Prism would crash when switching
between Prism and other apps after using a Prism script from the
GraphPad Prism Script dialog
 (Mac) Fixed the issue in which Prism would crash while attempting
to run a script that opens a file with a name containing 255 characters
 (Mac) Fixed the issue in which Prism would crash when opening the
Nonlinear regression dialog if identically named .PZF and .PZT files
with custom equations were used in the Equations folder
 (Mac) Fixed the issue in which Prism would freeze while saving a
Prism project to LabArchives after logging in to a different LabArchives
account
 (Mac) Fixed the issue in which Prism would select an incorrect
range of sheets in the Navigator using the SHIFT modifier key in the
combined "Data+Results" view in gallery mode
 (Mac) Fixed the issue in which selected thumbnails did not
disappear in the gallery mode after deleting them from the "Search
Results" folder
 (Mac) Fixed the issue in which changes made using the "Format This
Violin" contextual menu on a violin plot did not propagate into the
"Format Graph" dialog
 (Mac) Fixed the issue in which it was not possible to edit "K"
values and the "Title" field in the "Parameters: Select and Transform"
dialog
 (Mac) Fixed the issue in which selection arrows were nonfunctional in the "Parameters: Simulate 2 x 2 contingency table" dialog
 (Mac) Fixed the issue in which decimal places were not
automatically determined in a target table after pasting data with a
link from a green results sheet to a data table
 (Mac) Fixed the issue in which the number of subcolumns in the
"Format Data Table" dialog were lost after switching between table types
 (Mac) Fixed the issue in which a data table unexpectedly appeared
instead of a results sheet after clicking "OK" in analysis parameters
dialogs after inserting a new data table with a graph
 (Mac) Fixed the issue in which it was impossible to select the 95%
CI for Median and Error appearance in the "Format Graph" dialog for XY
graphs
 (Mac) Fixed the issue in which part of linked sheets were not
deleted from the Navigator after performing the "Delete Entire Family"
operation from the "Family" folder
 (Mac) Fixed the issue in which the "Family" folder in the Navigator
did not reappear after performing the "Undo" operation after an entire
family had been deleted
 (Mac) Fixed the issue with syncing options when the wrong pattern
style for a bar was shown in the "Format Graph" dialog after the pattern
was previously changed using the contextual menu on a graph
 (Mac) Fixed the issue in which thumbnails with results sheets
unexpectedly appeared in the Navigator after performing the "Delete
entire Family" command in gallery mode
 (Mac) Fixed the issue in which the name of the first
subcolumn appeared in a data table instead of "Mean" after a Nested data
table was changed to an XY or Grouped data table with error bars
calculated elsewhere
 (Mac) Fixed the alignment of an indication on the bottom status bar
of the currently selected cell in "Interpolated Y values"/"Interpolated
X values" results of the "Fit Spline/LOWESS" analysis
 (Mac) Fixed the corrupted hint in the annotation section of the RM
Design tab in "Parameters: Twoway ANOVA" dialog when "Correct for
multiple comparisons by controlling the False Discovery Rate" test was
selected
 (Mac) Fixed the issue in which the "Saved example" tab appeared
empty after clicking the "Back" button in the "Clone Example" dialog if
any graph was cloned previously
 (Mac) Fixed the issue in which the "Script Log" tab on the
"GraphPad Prism Script" dialog became greyed out when a script error
occurs
 (Mac) Fixed the issue in which the "Above Bar & Error Bar"
subtab did not appear highlighted in the "Annotations" tab of the
"Format Graph" dialog
 (Mac) Fixed the issue in which Prism would paste data incorrectly
after selecting the "After rows, start a new column" checkbox in the
"Import and Paste Special" dialog
Discover What's New in Prism 8!
Introducing an even more powerful Prism featuring enhanced data
visualization and graph customization, more intuitive navigation, and
more sophisticated statistical analyses.
Enhanced Data Visualization

Violin plots
Visualize distributions of large data sets more clearly than with boxwhisker or simple bar graphs


Subcolumn graphs
Organize related subsets of nested data in a single graph


Smoothing spline
Major improvements in showing general data trends through
Akima splines and smoothing splines with improved control over the
number of knots, or
inflection points


No more smiles
More intelligent adjustments of data point positions in scatter plots for better looking graphs

Read More
Improved Graphing and Customization Options

Draw lines and brackets with centered text
Easily annotate your data with asterisks or custom labels


Automatically label bar graphs
Annotate your bar graphs with values for the means, medians, or sample sizes to emphasize what's important in your work


Improved grouped graphs
Easily create graphs that show both individual points (scatter) along with bars for mean (or median) and error bars

Read More
More Intuitive Navigation
Find related sheets easily
New family panel shows the family of sheets related to the
current sheet, and chains of analyses are automatically indented
Easily navigate between multiple results tables
Analyses with multiple results tables now grouped into a
single sheet with tabs for each result table; choose which tabs to show
or hide
Improved Search
Search by sheets with highlights or notes of specified color
Now Featuring Eight Kinds of Data Tables
New: Multiple variables data table
Each row represents a
different subject and each column is a different variable, allowing you
to perform Multiple linear
regression (including Poisson regression), extract subsets
of data into other table types, or select and transform subsets of the
data
New: Nested data table
Analyze and visualize data that contains subsets within related groups; Perform nested t tests and nested oneway ANOVA
using data within these tables
More Sophisticated Statistical Analyses
Perform repeated measures ANOVA  even with missing data
Now Prism will automatically fit a mixed effects model to complete this analysis
Powerful Improvements in regular ANOVA
View cell, row, column, and grand means (or least square
means when data is missing); test for homogeneity of variance. For
oneway ANOVA, choose a test
that does not assume homogeneous variances.
Nested t test and nested oneway ANOVA
Utilize new types of data tables to perform nested ttests
and nested ANOVA as well as multiple linear regression (including
Poisson regression)
Graph residuals from multiple types of analyses
Test residuals for normality in four different ways, and choose from four different ways to display these residuals
Discover the Breadth of Statistical Features Available in Prism 8
Statistical Comparisons

Paired or unpaired t tests. Reports P values and confidence intervals.

Automatically generate volcano plot (difference vs. P value) from multiple t test analysis.

Nonparametric MannWhitney test, including confidence interval of difference of medians.

KolmogorovSmirnov test to compare two groups.

Wilcoxon test with confidence interval of median.

Perform many t tests at once, using False Discovery Rate (or
Bonferroni multiple comparisons) to choose which comparisons are
discoveries to study further.
 Ordinary or repeated measures ANOVA followed by the Tukey,
NewmanKeuls, Dunnett, Bonferroni or HolmSidak multiple comparison
tests, the posttest for trend, or
Fisher's Least Significant tests.

Oneway ANOVA without assuming populations with equal standard
deviations using BrownForsythe and Welch ANOVA, followed by appropriate
comparisons tests
(GamesHowell, Tamhane T2, Dunnett T3)

Many multiple comparisons test are accompanied by confidence intervals and multiplicity adjusted P values.

GreenhouseGeisser correction so repeated measures one, two,
and threeway ANOVA do not have to assume sphericity. When this is
chosen, multiple comparison tests
also do not assume sphericity.

KruskalWallis or Friedman nonparametric oneway ANOVA with Dunn's post test.

Fisher's exact test or the chisquare test. Calculate the relative risk and odds ratio with confidence intervals.

Twoway ANOVA, even with missing values with some post tests.
 Twoway ANOVA, with repeated measures in one or both factors.
Tukey, NewmanKeuls, Dunnett, Bonferroni, HolmSidak, or Fisher's LSD
multiple comparisons testing
main and simple effects.

Threeway ANOVA (limited to two levels in two of the factors, and any number of levels in the third).

Analysis of repeated measures data (one, two, and threeway)
using a mixed effects model (similar to repeated measures ANOVA, but
capable of handling missing
data).

KaplanMeier survival analysis. Compare curves with the logrank test (including test for trend).

Comparison of data from nested data tables using nested t test or nested oneway ANOVA (using mixed effects model).
Nonlinear Regression

Fit one of our 105 builtin equations, or enter your own. Now
including family of growth equations: exponential growth, exponential
plateau, Gompertz, logistic, and
beta (growth and then decay).

Enter differential or implicit equations.

Enter different equations for different data sets.
 Global nonlinear regression  share parameters between data sets.

Robust nonlinear regression.

Automatic outlier identification or elimination.

Compare models using extra sumofsquares F test or AICc.

Compare parameters between data sets.

Apply constraints.

Differentially weight points by several methods and assess how well your weighting method worked.

Accept automatic initial estimated values or enter your own.

Automatically graph curve over specified range of X values.

Quantify precision of fits with SE or CI of parameters.
Confidence intervals can be symmetrical (as is traditional) or
asymmetrical (which is more accurate).
 Quantify symmetry of imprecision with Hougaard's skewness.

Plot confidence or prediction bands.

Test normality of residuals.

Runs or replicates test of adequacy of model.

Report the covariance matrix or set of dependencies.

Easily interpolate points from the best fit curve.

Fit straight lines to two data sets and determine the intersection point and both slopes.
Column Statistics

Calculate descriptive statistics: min, max, quartiles, mean, SD, SEM, CI, CV, skewness, kurtosis.

Mean or geometric mean with confidence intervals.

Frequency distributions (bin to histogram), including cumulative histograms.

Normality testing by four methods (new: AndersonDarling).

Lognormality test and likelihood of sampling from normal (Gaussian) vs. lognormal distribution.

Create QQ Plot as part of normality testing.

One sample t test or Wilcoxon test to compare the column mean (or median) with a theoretical value.

Identify outliers using Grubbs or ROUT method.
 Analyze a stack of P values, using Bonferroni multiple
comparisons or the FDR approach to identify "significant" findings or
discoveries.
Linear Regression and Correlation

Calculate slope and intercept with confidence intervals

Force the regression line through a specified point.

Fit to replicate Y values or mean Y.

Test for departure from linearity with a runs test.

Calculate and graph residuals in four different ways (including QQ plot).

Compare slopes and intercepts of two or more regression lines.

Interpolate new points along the standard curve.

Pearson or Spearman (nonparametric) correlation.

Multiple linear regression (including Poisson regression) using the new multiple variables data table.
Clinical (Diagnostic) Lab Statistics

BlandAltman plots.

Receiver operator characteristic (ROC) curves.

Deming regression (type ll linear regression).
Simulations

Simulate XY, Column or Contingency tables.

Repeat analyses of simulated data as a MonteCarlo analysis.

Plot functions from equations you select or enter and parameter values you choose.
Other Calculations

Area under the curve, with confidence interval.

Transform data.

Normalize.

Identify outliers.

Normality tests.

Transpose tables.

Subtract baseline (and combine columns).

Compute each value as a fraction of its row, column or grand total.
Prism 8 for Windows
 Runs under either the 32 or 64bit versions of Windows Vista, 7, 8 or 10. The installer will detect your version of Windows and install the 32 or 64bit version of Prism accordingly.
 Requires a display resolution of at least 800 x 540 pixels. So the lowest standard display resolution for Prism is 800 x 600.
 Requires about 100 MB (megabytes) of space on the hard drive.
 Must connect to the internet to verify the license when it is first activated and also at at least once every 30 days (or every 20 launches, whichever comes sooner).
Prism 8 for Mac
 Runs under macOS X 10.9 (Mavericks) or later. If you use macOS 10.8, Prism will launch and seems to run OK, but we haven’t tested Prism thoroughly under this version of macOS and can’t provide much support. If you use 10.8, we urge you to update MacOS.
 Note: Prism on Mac OS Catalina (10.15): https://www.graphpad.com/support/faqid/2161/
 Requires a display with a resolution of at least 1024 x 768 pixels.
 Requires about 130 MB (megabytes) of space on the hard drive.
 Must connect to the internet to verify the license when it is first activated and also at at least once every 30 days (or every 20 launches, whichever comes sooner).
